Difference between revisions of "Part:BBa K4365002"

 
 
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<partinfo>BBa_K4365002 short</partinfo>
 
<partinfo>BBa_K4365002 short</partinfo>
  
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Origin of replication compatible with yeast. Could be used to clone into a bacterial plasmid to make it applicable for yeast expression.
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2 micron Ori
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The 2 micron ori of Saccharomyces cerevisiae is a multi-copy DNA element that resides in the yeast nucleus at a copy number of 40-60 per haploid cell <ref>Chan KM, Liu YT, Ma CH, Jayaram M, Sau S. The 2 micron plasmid of Saccharomyces cerevisiae: a miniaturized selfish genome with optimized functional competence. Plasmid. 2013;70(1):2-17. doi:10.1016/j.plasmid.2013.03.001</ref>. The plasmid is able to persist in host populations with almost chromosome-like stability.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:53, 11 October 2022


2 micron Ori

2 micron Ori The 2 micron ori of Saccharomyces cerevisiae is a multi-copy DNA element that resides in the yeast nucleus at a copy number of 40-60 per haploid cell [1]. The plasmid is able to persist in host populations with almost chromosome-like stability.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 360
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 360
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 360
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. ↑ Chan KM, Liu YT, Ma CH, Jayaram M, Sau S. The 2 micron plasmid of Saccharomyces cerevisiae: a miniaturized selfish genome with optimized functional competence. Plasmid. 2013;70(1):2-17. doi:10.1016/j.plasmid.2013.03.001