Difference between revisions of "Part:BBa K174004"

Revision as of 15:13, 10 October 2009

Pspac promoter

This promoter can be induced by IPTG and it is tightly regulated by LacI. Wild type lac operator is replaced with a perfect palindromic "oid" operator sequence which increases the repression by LacI more than 2.5 fold. To repress the promoter LacI needs to be expressed. Addition of IPTG will derepress LacI activity.

This biobrick is an enhancement of Biobrick BBa_K090501 which has the sequence from pmutin2. BBa_K143015 and BBa_I746665 biobricks have also the wild type lac operator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

Vagner, V., E. Dervyn, et al. (1998). "A vector for systematic gene inactivation in Bacillus subtilis." Microbiology 144(11): 3097-3104.
Lehming, N. et al. The interaction of the recognition helix of Lac repressor with lac operator. EMBO J. 6, 3145−3153 (1987).

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K174004 short</partinfo>
-This promoter can be induced by IPTG and it is tightly regulated by LacI. Wild type lac operator is replaced with a perfect palindromic "oid" operator sequence which increases the repression by LacI more than 2.5 fold and provide a To repress the promoter LacI needs to be expressed. Addition of IPTG will derepress LacI activity.
+This promoter can be induced by IPTG and it is tightly regulated by LacI. Wild type lac operator is replaced with a perfect palindromic "oid" operator sequence which increases the repression by LacI more than 2.5 fold. To repress the promoter LacI needs to be expressed. Addition of IPTG will derepress LacI activity.
 This biobrick is an enhancement of Biobrick BBa_K090501 which has the sequence from pmutin2. BBa_K143015 and BBa_I746665 biobricks have also the wild type lac operator.