Difference between revisions of "Part:BBa K4156126"

 
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HlpA is a binding protein from Streptococcus gallolyticus that binds to acetyl heparan sulfate glycoprotein (HSPG) on the tumor surface (specifically to syndecan 1, which is upregulated during carcinogenesis). HlpA has been shown to enhance the ability of engineered bacteria to penetrate colorectal tumor cells.
 
HlpA is a binding protein from Streptococcus gallolyticus that binds to acetyl heparan sulfate glycoprotein (HSPG) on the tumor surface (specifically to syndecan 1, which is upregulated during carcinogenesis). HlpA has been shown to enhance the ability of engineered bacteria to penetrate colorectal tumor cells.
  
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===Usage and Biology===
 
===Usage and Biology===
  
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===Characterization===
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To demonstrate that HlpA enables better bacterial adhesion to cells, we constructed HlpA with mRFP to characterize HlpA. And characterization details can be found at<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156128"> BBa_K4156128 </a></html>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4156126 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4156126 SequenceAndFeatures</partinfo>

Revision as of 14:21, 11 October 2022


HlpA with INP tag

HlpA is a binding protein from Streptococcus gallolyticus that binds to acetyl heparan sulfate glycoprotein (HSPG) on the tumor surface (specifically to syndecan 1, which is upregulated during carcinogenesis). HlpA has been shown to enhance the ability of engineered bacteria to penetrate colorectal tumor cells.

Usage and Biology

Characterization

To demonstrate that HlpA enables better bacterial adhesion to cells, we constructed HlpA with mRFP to characterize HlpA. And characterization details can be found at BBa_K4156128


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 330
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 72
    Illegal NgoMIV site found at 154
    Illegal NgoMIV site found at 405
    Illegal AgeI site found at 733
  • 1000
    COMPATIBLE WITH RFC[1000]