Difference between revisions of "Part:BBa K4156118"
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We introduced a dual vector system in the same strain. The XOR gate-mRFP is located on pSB4C5, while its upstream promoter and corresponding TP901/BxB1 integrase are located on another plasmid, pSB1A3, which are resistant to chloramphenicol and ampicillin, respectively. Through the expression of red fluorescent protein in the dual vector system, we can monitor the change of red fluorescent signal of the recombinant vector to test the performance of XOR gate.
 
We introduced a dual vector system in the same strain. The XOR gate-mRFP is located on pSB4C5, while its upstream promoter and corresponding TP901/BxB1 integrase are located on another plasmid, pSB1A3, which are resistant to chloramphenicol and ampicillin, respectively. Through the expression of red fluorescent protein in the dual vector system, we can monitor the change of red fluorescent signal of the recombinant vector to test the performance of XOR gate.
  
 +
===Characterization===
 +
We used three different promoter combinations with XOR gate-mRFP to characterize the performance of the promoter and TP901-coupled logic gates, as well as the effect of the addition of lysis genes.
 +
 +
Characterization details can be found at：
 +
pCadC-TP901:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156112"> BBa_K4156112 </a></html>
 +
pCadC-TP901-φ174E:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156112"> BBa_K4156112 </a></html>
 +
pLldR-TP901:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156112"> BBa_K4156112 </a></html>
 +
pLldR-TP901-φ174E:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156112"> BBa_K4156112 </a></html>
 +
pPepT-TP901:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156112"> BBa_K4156112 </a></html>
 +
pPepT-TP901-φ174E:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156112"> BBa_K4156112 </a></html>
  
  

Revision as of 14:16, 11 October 2022


XOR gate-mRFP

XOR gate-mRFP consists of an XOR logic gate ( BBa_K4156117 ) and a downstream RFP sequence to test the performance of the XOR gate by detecting the red fluorescent signal.


Usage and Biology

We introduced a dual vector system in the same strain. The XOR gate-mRFP is located on pSB4C5, while its upstream promoter and corresponding TP901/BxB1 integrase are located on another plasmid, pSB1A3, which are resistant to chloramphenicol and ampicillin, respectively. Through the expression of red fluorescent protein in the dual vector system, we can monitor the change of red fluorescent signal of the recombinant vector to test the performance of XOR gate.

Characterization

We used three different promoter combinations with XOR gate-mRFP to characterize the performance of the promoter and TP901-coupled logic gates, as well as the effect of the addition of lysis genes.

Characterization details can be found at： pCadC-TP901: BBa_K4156112 pCadC-TP901-φ174E: BBa_K4156112 pLldR-TP901: BBa_K4156112 pLldR-TP901-φ174E: BBa_K4156112 pPepT-TP901: BBa_K4156112 pPepT-TP901-φ174E: BBa_K4156112


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 104
    Illegal AgeI site found at 1097
    Illegal AgeI site found at 1209
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 124
    Illegal BsaI.rc site found at 351