Difference between revisions of "Part:BBa K4156112"

Latest revision as of 13:54, 11 October 2022

plac-LuxCDABE

plac-LuxCDABE is a complex regulatory system of plac operon ( BBa_R0010 ) and LuxCDABE operon We designed plac-LuxCDABE as a control for observing the abundance level of bacteria.

Usage and Biology

In this biobrick, we fused the plac( BBa_K4156079 ) with the LuxCDABE ( BBa_K4156095 ) and introduced the RiboJ sequence and RBS B0034 in between. this biobrick was used to facilitate the observation of bacterial abundance changes in tumor cells while avoiding the environmental interference associated with the use of mRFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 328
Illegal BglII site found at 2309
Illegal BglII site found at 5561
Illegal BamHI site found at 872
Illegal BamHI site found at 5861
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 741
Illegal NgoMIV site found at 1368
Illegal AgeI site found at 5879
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4156112 short</partinfo>
-plac-LuxCDABE is a complex regulatory system of plac operon (BBa_R0010) and LuxCDABE operon We designed plac-LuxCDABE as a control for observing the abundance level of bacteria.
+plac-LuxCDABE is a complex regulatory system of plac operon (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_R0010"> BBa_R0010 </a></html> ) and LuxCDABE operon We designed plac-LuxCDABE as a control for observing the abundance level of bacteria.
@@ Line 10: / Line 10: @@
 ===Usage and Biology===
-In this biobrick, we fused the plac operon with the LuxCDABE operon and introduced the RiboJ sequence and RBS B0034 in between. this biobrick was used to facilitate the observation of bacterial abundance changes in tumor cells while avoiding the environmental interference associated with the use of mRFP.
+In this biobrick, we fused the plac(<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:	BBa_K4156079"> 	BBa_K4156079 </a></html> )  with the LuxCDABE (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:	BBa_K4156095"> 	BBa_K4156095 </a></html> )  and introduced the RiboJ sequence and RBS B0034 in between. this biobrick was used to facilitate the observation of bacterial abundance changes in tumor cells while avoiding the environmental interference associated with the use of mRFP.