Difference between revisions of "Part:BBa K4129120"
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===Usage and Biology===
 
===Usage and Biology===
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This composite part was used to characterise the synthetic transcription factor (sTF) FunsTF01. The reporter cassette consists of the synthetic 6xLexO-Pmin (BBa_K4129120) that transcribe mCherry.
  
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Revision as of 13:51, 11 October 2022


The fungal synthetic expression system with FunsTF01 and 6xLexO-Pmin


Usage and Biology

This composite part was used to characterise the synthetic transcription factor (sTF) FunsTF01. The reporter cassette consists of the synthetic 6xLexO-Pmin (BBa_K4129120) that transcribe mCherry.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4174
    Illegal XbaI site found at 6087
    Illegal PstI site found at 141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 911
    Illegal NheI site found at 2986
    Illegal PstI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 145
    Illegal BamHI site found at 2920
    Illegal BamHI site found at 3512
    Illegal BamHI site found at 5368
    Illegal XhoI site found at 701
    Illegal XhoI site found at 1083
    Illegal XhoI site found at 3661
    Illegal XhoI site found at 4038
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4174
    Illegal XbaI site found at 6087
    Illegal PstI site found at 141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4174
    Illegal XbaI site found at 6087
    Illegal PstI site found at 141
    Illegal NgoMIV site found at 3078
    Illegal NgoMIV site found at 6004
    Illegal AgeI site found at 1290
    Illegal AgeI site found at 1438
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1117
    Illegal BsaI.rc site found at 5718
    Illegal SapI site found at 323