Difference between revisions of "Part:BBa K4385011"
Line 7: Line 7:
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
−
===Characterization===
+
 
−
To verify the inhibitory effect of lacI on Ptrc, two genetic circuits were constructed (Fig1). The trends of fluorescence intensity were measured at the indicated time. As shown in the Fig1, the fluorescence of the experimental group was suppressed and stabilized at a low value, while that of the control group increased with time. It demonstrated that Ptrc is normally a strong constitutive promoter, while the Lac repressor encoded by lacI has a significant inhibitor on Ptrc.
+
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 13:43, 11 October 2022


Lead sensor

The lead sensor is composed of lead-inducible promoter and GFP. When there is no lead ion in the environment, PbrR protein is expressed in the presence of Pbr constitutive promoter and binds to its downstream inducible promoter Pbc to repress GFP expression; when lead ion reaches a certain concentration in the environment, PbrR protein chelates lead ion so that it cannot bind to the inducible promoter Pbc and downstream GFP can be expressed and the engineered bacteria can see green fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 462
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 462
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 462
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 253
    Illegal BsaI.rc site found at 1394