Difference between revisions of "Part:BBa K4376001"
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Its encoding protein is blue-pigment indigoidine synthetase, which carries strong fluxes toward L-glutamate, a precursor of indigoidine. In our project, we sue it to produce indigoidine. | Its encoding protein is blue-pigment indigoidine synthetase, which carries strong fluxes toward L-glutamate, a precursor of indigoidine. In our project, we sue it to produce indigoidine. | ||
| − | We amplified two bpsA fragments then transformed recombinant vector into DH5α and Corynebacterium glutamicum. | + | We amplified two bpsA fragments then transformed recombinant vector into DH5α E.coli strain and Corynebacterium glutamicum. |
[[File:engineering-1.png|600px||centre]] | [[File:engineering-1.png|600px||centre]] | ||
Revision as of 13:35, 11 October 2022
Produce indigoidine
Its encoding protein is blue-pigment indigoidine synthetase, which carries strong fluxes toward L-glutamate, a precursor of indigoidine. In our project, we sue it to produce indigoidine.
We amplified two bpsA fragments then transformed recombinant vector into DH5α E.coli strain and Corynebacterium glutamicum.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3887
Illegal XhoI site found at 3040
Illegal XhoI site found at 3739 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 286
Illegal NgoMIV site found at 693
Illegal AgeI site found at 1951
Illegal AgeI site found at 2004 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 229
Illegal BsaI site found at 1540
Illegal BsaI site found at 2254
Illegal BsaI.rc site found at 358
Illegal BsaI.rc site found at 3544
Illegal SapI.rc site found at 1792