Difference between revisions of "Part:BBa K4376003"

Revision as of 13:35, 11 October 2022

the composite part is used to express the bpsA gene.

Construction of the bpsA gene and tac inducible promoter to control the production of the blue-pigment indigoidine synthetase. The composite part is inserted into C.glutamicum in plasmid, resulting in the successful biosynthesis of indigodine pigment.

We amplified two bpsA fragments then transformed recombinant vector into DH5α E.coli strain and Corynebacterium glutamicum.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3893
Illegal XhoI site found at 3046
Illegal XhoI site found at 3745
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 292
Illegal NgoMIV site found at 699
Illegal AgeI site found at 1957
Illegal AgeI site found at 2010
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 235
Illegal BsaI site found at 1546
Illegal BsaI site found at 2260
Illegal BsaI.rc site found at 364
Illegal BsaI.rc site found at 3550
Illegal SapI.rc site found at 1798

@@ Line 5: / Line 5: @@
 Construction of the bpsA gene and tac inducible promoter to control the production of the blue-pigment indigoidine synthetase. The composite part is inserted into C.glutamicum in plasmid, resulting in the successful biosynthesis of indigodine pigment.
-We amplified two bpsA fragments then transformed recombinant vector into DH5αE.coli strain and Corynebacterium glutamicum.
+We amplified two bpsA fragments then transformed recombinant vector into DH5α E.coli strain and Corynebacterium glutamicum.
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