Difference between revisions of "Part:BBa K4388005:Experience"

 
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Through JUMP type IIS Level 1, this transcriptional unit (BBa_K4388005) was assembled in a one-pot digestion reaction with BsaI and ligation into pJUMP29-1A. Successful ligation and transformation were selected for by identifying non-fluorescent colonies on kanamycin agar plates (Fig. 1).
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[[File:Level_1_Results_TU1_(K4388005).png]]
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Figure 1. Construction of Transcriptional Unit (TU) BBa_K4388005 into Level 1 pJUMP29-1A vector. Level 0 parts BBa_K4388000, BBa_K4388001, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388005 in pJUMP29-1A via a one-pot BsaI digestion reaction. White (sfGPF-excised) colonies indicate successful ligation of the TU into plasmid, and successful transformation of plasmid conferring kanamycin resistance. Final level 1 plasmid constructs were designed using SnapGene.
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Revision as of 13:17, 11 October 2022


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4388005

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UNIQ0743d12dcc9a0e7f-partinfo-00000000-QINU UNIQ0743d12dcc9a0e7f-partinfo-00000001-QINU