Difference between revisions of "Part:BBa K4461013:Experience"

(→‎Applications of BBa_K4461013)
(→‎Applications of BBa_K4461013)
 
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If the plasmid contained glpABC-mCherry with tag, there will be an EcoRI site that we used to insert tag by enzyme digestion and ligation, or it is a plasmid contained mut-glpABC-mCherry, which doesn't have an EcoRI site on it. Therefore, we digested them with EcoRI and BamHI, and the one being digested into 2 bands is correct.
 
If the plasmid contained glpABC-mCherry with tag, there will be an EcoRI site that we used to insert tag by enzyme digestion and ligation, or it is a plasmid contained mut-glpABC-mCherry, which doesn't have an EcoRI site on it. Therefore, we digested them with EcoRI and BamHI, and the one being digested into 2 bands is correct.
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[[File:Glp-mCherry-tag-check.jpg|400px|thumb|center|Fig1. After EcoRI/BamHI enzyme digestion, we found the correct plasmid (glpABC-mCherry-tag1). ]]
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[[File:Glp-mCherry-tag-check.jpg|200px|thumb|center|Fig1. After EcoRI/BamHI enzyme digestion, we found the correct plasmid (glpABC-mCherry-tag1). ]]
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The fusion protein are tested under the glpABC promoter and compared with that without tag.
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[[File:Degredation tag test.png|300px|thumb|center| Fig.2 The result that we measured the FP intensity and OD600 for the mCherry with and without tag(BBa_K4461505 and BBa_K4461504). ]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 12:46, 11 October 2022


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4461013

If the plasmid contained glpABC-mCherry with tag, there will be an EcoRI site that we used to insert tag by enzyme digestion and ligation, or it is a plasmid contained mut-glpABC-mCherry, which doesn't have an EcoRI site on it. Therefore, we digested them with EcoRI and BamHI, and the one being digested into 2 bands is correct.

Fig1. After EcoRI/BamHI enzyme digestion, we found the correct plasmid (glpABC-mCherry-tag1).

The fusion protein are tested under the glpABC promoter and compared with that without tag.

Fig.2 The result that we measured the FP intensity and OD600 for the mCherry with and without tag(BBa_K4461505 and BBa_K4461504).

User Reviews

UNIQ8976c282f921d940-partinfo-00000000-QINU UNIQ8976c282f921d940-partinfo-00000001-QINU