Difference between revisions of "Part:BBa K4461013"
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<partinfo>BBa_K4461013 short</partinfo> | <partinfo>BBa_K4461013 short</partinfo> | ||
− | We synthesized a commercial sequence of tag (BBa_K1051207) and EcoRI and HindIII cutting sites on its prefix and suffix. Therefore, we can insert tag into our construct (BBa_K4461010) by restriction enzyme digestion. | + | An improved alternative to mCherry (BBa_J18932) that reduces the fluorescence intensity by fusion with tag(BBa_K1051207). |
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+ | We synthesized a commercial sequence of tag (BBa_K1051207) and EcoRI and HindIII cutting sites on its prefix and suffix. Therefore, we can insert the tag into our construct (BBa_K4461010) by restriction enzyme digestion. | ||
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Revision as of 12:39, 11 October 2022
mCherry+tag fusion protein
An improved alternative to mCherry (BBa_J18932) that reduces the fluorescence intensity by fusion with tag(BBa_K1051207).
We synthesized a commercial sequence of tag (BBa_K1051207) and EcoRI and HindIII cutting sites on its prefix and suffix. Therefore, we can insert the tag into our construct (BBa_K4461010) by restriction enzyme digestion.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 694
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 694
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 694
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 694
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 694
- 1000COMPATIBLE WITH RFC[1000]