Difference between revisions of "Part:BBa K243002:Design"

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===References===
 
===References===
  
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Steiner, D; Forrer, P; Stumpp, MT,(2006).Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display ''NATURE BIOTECHNOLOGY''Vol.24 Issue:7 Pages: 823-831
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Steiner, D; Forrer, P; Stumpp, MT,(2006).Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display ''NATURE BIOTECHNOLOGY'' Vol.24 Issue:7 Pages: 823-831

Revision as of 15:54, 8 October 2009

DsbA signal sequence (enables periplasm export)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the signal sequence had to be located at the N-terminus of the (fusion)protein.


Source

Sequence of the DsbA was copied out from E.coli B genom Synthesized oligos with signal sequence of DsbA by sigma.

References

Steiner, D; Forrer, P; Stumpp, MT,(2006).Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display NATURE BIOTECHNOLOGY Vol.24 Issue:7 Pages: 823-831