Difference between revisions of "Part:BBa K4165235"
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<partinfo>BBa_K4165235 short</partinfo>
 
<partinfo>BBa_K4165235 short</partinfo>
  
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This biobrick consists of T7 promotor (BBa_K3633015), RBS (BBa_K4165016), 6-His tag (BBa_K4165020), UBE2W (BBa_K4165015), T7 Terminator (BBa_K731721), The His tag was attached to the UBE2W coding sequence to serve in the purification using NI-NTA column.
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This biobrick consists of T7 promotor (BBa_K3633015), RBS 5 (BBa_K4165075), 6-His tag (BBa_K4165020), UBE2W (BBa_K4165015), T7 Terminator (BBa_K731721), The His tag was attached to the UBE2W coding sequence to serve in the purification using NI-NTA column.
  
 
===Source===
 
===Source===

Revision as of 12:31, 11 October 2022


His - UBE2W

This biobrick consists of T7 promotor (BBa_K3633015), RBS 5 (BBa_K4165075), 6-His tag (BBa_K4165020), UBE2W (BBa_K4165015), T7 Terminator (BBa_K731721), The His tag was attached to the UBE2W coding sequence to serve in the purification using NI-NTA column.

Source

Synthesized

Usage and Biology

Ubiquitin-conjugating E2 ligase has a role in the ubiquitination cascade for protein degradation. It has been used in this project to target and degrade both tau and Aβ proteins which are both considered the main causes of Alzheimer’s Disease pathogenesis).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Dry lab

Mathematical Modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab. 

              Figure 1. this figure shows the results from the transcription and translation code showing the 
                                       the concentration of the protein with time.

WetLab Results

Transformation of His UBE 2w in BL-21 using pGS-21a vector 

                             Figure 2. Transformed plate of His UBE 2w + pGS-21a

Transformation of His UBE 2w in DH-5 alpha using pJET vector

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                             Figure 3. Transformed plate of His UBE 2w + pJET