Difference between revisions of "Part:BBa K243002:Design"
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+ | Author(s): Steiner, D; Forrer, P; Stumpp, MT,(2006).Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display ''NATURE BIOTECHNOLOGY''Vol.24 Issue:7 Pages: 823-831 |
Revision as of 15:53, 8 October 2009
DsbA signal sequence (enables periplasm export)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
the signal sequence had to be located at the N-terminus of the (fusion)protein.
Source
Sequence of the DsbA was copied out from E.coli B genom Synthesized oligos with signal sequence of DsbA by sigma.
References
Author(s): Steiner, D; Forrer, P; Stumpp, MT,(2006).Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display NATURE BIOTECHNOLOGYVol.24 Issue:7 Pages: 823-831