Difference between revisions of "Part:BBa K4273001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Xyl1 is a xylose assimilation gene encoding xylose reductase (XR) that can converts xylose to xylitol. Xyl1 can provides an alternative carbon source for the pentose phosphate pathway that can increase S7P production. We selected Xyl1 genes from Scheffersomyces stipitis that proved to be successfully heterologous expressed for efficiently xylose-fermenting in S. cerevisiae. We used yeast toolkit (Lee, 2015) to assemble the Level 1 plasmid including the promoter, coding sequence, and terminator. Xyl1 is under the control of promoter pTDH3 which provides a high and stable transcription efficiency (Apel, et. al, 2016). Then, we used PCR amplification to obtain the DNA fragment Xyl1 from Level1 plasmid, and two homology arms (LA and RA) from yeast genome. | Xyl1 is a xylose assimilation gene encoding xylose reductase (XR) that can converts xylose to xylitol. Xyl1 can provides an alternative carbon source for the pentose phosphate pathway that can increase S7P production. We selected Xyl1 genes from Scheffersomyces stipitis that proved to be successfully heterologous expressed for efficiently xylose-fermenting in S. cerevisiae. We used yeast toolkit (Lee, 2015) to assemble the Level 1 plasmid including the promoter, coding sequence, and terminator. Xyl1 is under the control of promoter pTDH3 which provides a high and stable transcription efficiency (Apel, et. al, 2016). Then, we used PCR amplification to obtain the DNA fragment Xyl1 from Level1 plasmid, and two homology arms (LA and RA) from yeast genome. | ||
Revision as of 12:10, 11 October 2022
xyl1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 89
Illegal BglII site found at 478 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 109
- 1000COMPATIBLE WITH RFC[1000]