Difference between revisions of "Part:BBa K243000:Design"

 
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===References===
 
===References===
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NUCLEOTIDE-SEQUENCE OF THE FOKI RESTRICTION-MODIFICATION SYSTEM - SEPARATE STRAND-SPECIFICITY DOMAINS IN THE METHYLTRANSFERASE Author(s): LOONEY, MC; MORAN, LS; JACK, WE, et al.GENE Vol.80 Issue:2 Pages:193-208 Published:AUG 15 1989

Revision as of 13:34, 8 October 2009

Protein domain (active) of the restriction endonuclease FokI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 487


Design Notes

Modifications of the vector (catalytic active heterodimer) -heterodimeric aminio acids 

  * switch Glutamate 490/310-312 to Lysin (GAA->AAA)

  * switch isoleucin 538/454-456 to Lysin (ATC->AAA)


Source

extract coding region of Fok from the restriction-modification genes of the chromosomal DNA of Flavobacterium okeanokoites. Part synthesized by Mr.Gene


References

NUCLEOTIDE-SEQUENCE OF THE FOKI RESTRICTION-MODIFICATION SYSTEM - SEPARATE STRAND-SPECIFICITY DOMAINS IN THE METHYLTRANSFERASE Author(s): LOONEY, MC; MORAN, LS; JACK, WE, et al.GENE Vol.80 Issue:2 Pages:193-208 Published:AUG 15 1989