Difference between revisions of "Part:BBa K238008"
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This is VirA, a histidine kinase sensor originally found in Agrobacterium tumefaciens, Ti plasmid. It is part of the two-component VirA/VirG signal transduction system. This system is able to sense phenolic compounds and regulate gene transcription correspondingly.  
 
This is VirA, a histidine kinase sensor originally found in Agrobacterium tumefaciens, Ti plasmid. It is part of the two-component VirA/VirG signal transduction system. This system is able to sense phenolic compounds and regulate gene transcription correspondingly.  
 +
 +
VirA has illegal PstI restriction sites in its sequence. We used site directed mutagenesis to get these sites out. PstI was mutated from CTGCAG to CTGCAA.
  
 
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Revision as of 12:44, 8 October 2009

VirA

This is VirA, a histidine kinase sensor originally found in Agrobacterium tumefaciens, Ti plasmid. It is part of the two-component VirA/VirG signal transduction system. This system is able to sense phenolic compounds and regulate gene transcription correspondingly.

VirA has illegal PstI restriction sites in its sequence. We used site directed mutagenesis to get these sites out. PstI was mutated from CTGCAG to CTGCAA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 502
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 666
    Illegal BglII site found at 1559
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1405
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1177
    Illegal BsaI site found at 1969
    Illegal SapI site found at 1651
    Illegal SapI.rc site found at 880