Difference between revisions of "Part:BBa K4164015:Design"
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
This sequence is composed of [https://parts.igem.org/Part:BBa_J64997 BBa_J64997], [https://parts.igem.org/Part:BBa_K4164995 BBa_K4164995], [https://parts.igem.org/Part:BBa_K2556021 BBa_K2556021], [https://parts.igem.org/Part:BBa_K4164997 BBa_K416997] and [https://parts.igem.org/Part:BBa_K923004 BBa_K923004].
+
This sequence is composed of [https://parts.igem.org/Part:BBa_J64997 BBa_J64997], [https://parts.igem.org/Part:BBa_K4164995 BBa_K4164995], [https://parts.igem.org/Part:BBa_K2556021 BBa_K2556021], [https://parts.igem.org/Part:BBa_K4164997 BBa_K4164997] and [https://parts.igem.org/Part:BBa_K923004 BBa_K923004].
  
 
===References===
 
===References===

Latest revision as of 11:55, 11 October 2022


Inductive expression of RXR-ddRFPB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1964
    Illegal NheI site found at 2232
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 454
    Illegal AgeI site found at 1481
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 836


Design Notes

We recombined BBa_K4164997 to plasmid pET-29a+ by homologous recombination.


Source

This sequence is composed of BBa_J64997, BBa_K4164995, BBa_K2556021, BBa_K4164997 and BBa_K923004.

References