Difference between revisions of "Part:BBa K4164015"
| Line 3: | Line 3: | ||
<partinfo>BBa_K4164015 short</partinfo> | <partinfo>BBa_K4164015 short</partinfo> | ||
| − | In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we also tried | + | In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we also tried T7<em>lac</em> promoter from pET-29a+(Novagen). |
The composite part can be directly imported into plasmid and express RXR-ddRFPA1 induced with IPTG. | The composite part can be directly imported into plasmid and express RXR-ddRFPA1 induced with IPTG. | ||
Revision as of 11:44, 11 October 2022
Inductive expression of RXR-ddRFPB1
In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we also tried T7lac promoter from pET-29a+(Novagen).
The composite part can be directly imported into plasmid and express RXR-ddRFPA1 induced with IPTG.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1964
Illegal NheI site found at 2232 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 454
Illegal AgeI site found at 1481 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 836