Difference between revisions of "Part:BBa K4239005:Design"

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===Design Notes===
 
===Design Notes===
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<p><i>fiatluxE</i> did not have any mutation compare to <i>iluxE</i>, because no iGEM restriction site (EcoR1, Xba1, Spe1 and Pst1) were into the gene.
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<p><i>iluxE</i> did not have any mutation compare to <i>luxE</i>, according to Gregor et al.’s study in 2018.</p>
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===Source===
 
===Source===
  
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<p>The source of <i>fiatluxA</i> was the <i>ilux</i> operon available in a pGEX plasmid. An overlap PCR was performed to reconstitute directly <i>fiatluxABE</i> fragments which had been cut by the restriction enzymes.</p>
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===References===
 
===References===
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Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.
 
Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.
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Revision as of 11:34, 11 October 2022


Enhanced luciferase substrate forming unit fiatluxE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 588
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

fiatluxE did not have any mutation compare to iluxE, because no iGEM restriction site (EcoR1, Xba1, Spe1 and Pst1) were into the gene.

iluxE did not have any mutation compare to luxE, according to Gregor et al.’s study in 2018.

===Source===

The source of fiatluxA was the ilux operon available in a pGEX plasmid. An overlap PCR was performed to reconstitute directly fiatluxABE fragments which had been cut by the restriction enzymes.

References

Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.