Difference between revisions of "Part:BBa K4317002"
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Figure 1. Expression of mScarlet by various promoter in Bacillus subtilis 1A976 and Escherichia coli BL21(de3) | Figure 1. Expression of mScarlet by various promoter in Bacillus subtilis 1A976 and Escherichia coli BL21(de3) | ||
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| + | The mglv proter did not work properly in our Bacillus subtilis expression system. Even without the addition of maltose, mScarlet expression was increased when grown for 24 hours. | ||
===References=== | ===References=== | ||
Latest revision as of 11:21, 11 October 2022
Pmglv
Maltose-inducible promoter usable in Bacillus subtilis
Usage and Biology
Figure 1. Expression of mScarlet by various promoter in Bacillus subtilis 1A976 and Escherichia coli BL21(de3)
The mglv proter did not work properly in our Bacillus subtilis expression system. Even without the addition of maltose, mScarlet expression was increased when grown for 24 hours.
References
Ming, Yang M., et al. "Development of a Bacillus subtilis expression system using the improved Pglv promoter." Microbial cell factories 9.1 (2010): 1-8. Song, Yafeng, et al. "High-efficiency secretion of β-mannanase in Bacillus subtilis through protein synthesis and secretion optimization." Journal of agricultural and food chemistry 65.12 (2017): 2540-2548.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 7
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 7
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 7
Illegal BglII site found at 13
Illegal BamHI site found at 1 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 7
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 7
- 1000COMPATIBLE WITH RFC[1000]