Difference between revisions of "Part:BBa K4317002"

Revision as of 11:18, 11 October 2022

Pmglv

Maltose-inducible promoter usable in Bacillus subtilis

Usage and Biology

Figure 1. Expression of mScarlet by various promoter in Bacillus subtilis 1A976 and Escherichia coli BL21(de3)

References

Ming, Yang M., et al. "Development of a Bacillus subtilis expression system using the improved Pglv promoter." Microbial cell factories 9.1 (2010): 1-8. Song, Yafeng, et al. "High-efficiency secretion of β-mannanase in Bacillus subtilis through protein synthesis and secretion optimization." Journal of agricultural and food chemistry 65.12 (2017): 2540-2548.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 7
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 7
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 7
Illegal BglII site found at 13
Illegal BamHI site found at 1
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 7
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 7
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Maltose-inducible promoter usable in Bacillus subtilis
-<!-- Add more about the biology of this part here
+https://static.igem.org/mediawiki/parts/thumb/f/f8/Pmglv.png/800px-Pmglv.png
 ===Usage and Biology===
+https://static.igem.org/mediawiki/parts/thumb/5/51/Promoter_strength.png/800px-Promoter_strength.png
+Figure 1. Expression of mScarlet by various promoter in Bacillus subtilis 1A976 and Escherichia coli BL21(de3)
+===References===
+Ming, Yang M., et al. "Development of a Bacillus subtilis expression system using the improved Pglv promoter." Microbial cell factories 9.1 (2010): 1-8.
+Song, Yafeng, et al. "High-efficiency secretion of β-mannanase in Bacillus subtilis through protein synthesis and secretion optimization." Journal of agricultural and food chemistry 65.12 (2017): 2540-2548.
 <!-- -->