Difference between revisions of "Part:BBa K4252001"
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==Result==
 
==Result==
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===Results of pET-22b(+)-yjbB sequencing (primers: YjbB-2-5′, YjbB-2-3′)
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<br>
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[[File: results-of-pet-22b-yjbB-sequencing.png|500px|thumb|center|Figure 1: results-of-pet-22b-yjbB-sequencing.]]

Revision as of 10:14, 11 October 2022


phosphate exportation

As a phosphorus export element.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 426
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1174


Introduction

So far, only one type of P exporting protein, YjbB, has been reported in E. coli.The mechanism of phosphorus export by yjbB is still unclear. But based on experimental phenomena we choose YjbB as the phosphorus output element in our project.

Engineering

DNA fragments containing yjbB was amplified from E. coli MG1655 genomic DNA using the primers yjbB-5’/yjbB-3’.The PCR fragments were inserted into the Xbal/Xhol sites of pET-22b(+).

Result

===Results of pET-22b(+)-yjbB sequencing (primers: YjbB-2-5′, YjbB-2-3′)

Figure 1: results-of-pet-22b-yjbB-sequencing.