Difference between revisions of "Part:BBa K4244042"

 
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<partinfo>BBa_K4244042 short</partinfo>
 
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This part is the active full-length recombinant human MMP-9, to be expressed and purified from E. coli. It consists of amino acids 107-707, mimicing the cleaved and activated MMP-9. It can be purified from E. coli using the terminal 6xHIS-tag, following instructions from Mohseni et al.
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This part is the active full-length recombinant human MMP-9, to be expressed and purified from ''E. coli''. It consists of amino acids 107-707, mimicing the cleaved and activated MMP-9. It can be purified from ''E. coli'' using the terminal 6xHIS-tag, following instructions from Mohseni et al.
  
 
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<partinfo>BBa_K4244042 parameters</partinfo>
 
<partinfo>BBa_K4244042 parameters</partinfo>
 
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===References===
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Mohseni, S., Moghadam, T. T., Dabirmanesh, B., & Khajeh, K. (2016). Expression, purification, refolding and in vitro recovery of active full length recombinant human gelatinase MMP-9 in Escherichia coli. Protein expression and purification, 126, 42-48.

Revision as of 10:06, 11 October 2022


Active recombinant human MMP-9

This part is the active full-length recombinant human MMP-9, to be expressed and purified from E. coli. It consists of amino acids 107-707, mimicing the cleaved and activated MMP-9. It can be purified from E. coli using the terminal 6xHIS-tag, following instructions from Mohseni et al.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 75
    Illegal BamHI site found at 56
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1456
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Mohseni, S., Moghadam, T. T., Dabirmanesh, B., & Khajeh, K. (2016). Expression, purification, refolding and in vitro recovery of active full length recombinant human gelatinase MMP-9 in Escherichia coli. Protein expression and purification, 126, 42-48.