Difference between revisions of "Part:BBa K4244037"

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<partinfo>BBa_K4244037 short</partinfo>
 
<partinfo>BBa_K4244037 short</partinfo>
  
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The medium promoter from Wenk et al. was combined with an RBS derived from RBS calculator. In between these a target region was introduced including PAM and the t5 protospacer was introduced, which is targeted by dSpyCas9.
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The medium promoter from Wenk et al. was combined with an RBS derived from [https://salislab.net/software/predict_rbs_calculator RBS calculator]<br> In between these a target region was introduced including PAM and the t5 protospacer was introduced, which is targeted by dSpyCas9.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:02, 11 October 2022


Medium promoter + medium RBS

The medium promoter from Wenk et al. was combined with an RBS derived from RBS calculator
In between these a target region was introduced including PAM and the t5 protospacer was introduced, which is targeted by dSpyCas9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Wenk, S., Yishai, O., Lindner, S. N., & Bar-Even, A. (2018). An Engineering Approach for Rewiring Microbial Metabolism. In Methods in Enzymology (Vol. 608, pp. 329–367). Academic Press Inc. https://doi.org/10.1016/bs.mie.2018.04.026