Difference between revisions of "Part:BBa K4244006"

Revision as of 09:50, 11 October 2022

OmpC

OmpC is a porin gene, whose promoter is controlled by the transcription factor OmpR part of the EnvZ/OmpR two component system. The EnvZ/OmpR two-component system in normal conditions controls the expression of the ompC and ompF porin genes, controlling iron homeostasis (1). Being this part native of E.coli, it has directly been amplified from the organism that we are working on: E.coli Nissle 1917. Since this part originally upstream the OmpC gene, it also contains its native rbs. E.coli OmpC without rbs is under the iGEM registry code BBa_K769001.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

1. Gerken H, Vuong P, Soparkar K, Misra R. Roles of the EnvZ/OmpR Two-Component System and Porins in Iron Acquisition in Escherichia coli. mBio. 2020;11(3):e01192-20. Published 2020 Jun 23. doi:10.1128/mBio.01192-20

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 <partinfo>BBa_K4244006 short</partinfo>
-OmpC is a porin gene, whose promoter is controlled by the transcription factor OmpR part of the EnvZ/OmpR two component system. The EnvZ/OmpR two-component system in normla conditions control expression of the ompC and ompF porin genes, controlling iron homeostasis (1). Being this part native of E.coli, it has directly been amplified from the organism that we are working on: E.coli Nissle 1917. Since this part originally upstream the OmpC gene, it also contains its native rbs. E.coli OmpC without rbs is under the iGEM registry code <partinfo>BBa_K769001</partinfo>.
+OmpC is a porin gene, whose promoter is controlled by the transcription factor OmpR part of the EnvZ/OmpR two component system. The EnvZ/OmpR two-component system in normal conditions controls the expression of the ompC and ompF porin genes, controlling iron homeostasis (1). Being this part native of E.coli, it has directly been amplified from the organism that we are working on: ''E.coli'' Nissle 1917. Since this part originally upstream the OmpC gene, it also contains its native rbs. E.coli OmpC without rbs is under the iGEM registry code <partinfo>BBa_K769001</partinfo>.
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