Difference between revisions of "Part:BBa K4335022"
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The resistance gene APHVII<a href="https://parts.igem.org/Part:BBa_K4335003">[BBa_K4335003]</a>  was optimized for Chlamydomonas reinhardtii on the basis of the progene. We inserted a 145 BP intron into the progene to facilitate its expression in Chlamydomonas reinhardtii.
 
The resistance gene APHVII<a href="https://parts.igem.org/Part:BBa_K4335003">[BBa_K4335003]</a>  was optimized for Chlamydomonas reinhardtii on the basis of the progene. We inserted a 145 BP intron into the progene to facilitate its expression in Chlamydomonas reinhardtii.
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 09:28, 11 October 2022


Rbcs2 Promotor+ChlamyHgR+Rbcs2 Term

BBa K4335022 consists of a promoter, a resistance gene, and a terminator. The resistance gene APHVII[BBa_K4335003] was optimized for Chlamydomonas reinhardtii on the basis of the progene. We inserted a 145 BP intron into the progene to facilitate its expression in Chlamydomonas reinhardtii. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1236
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1121
  • 1000
    COMPATIBLE WITH RFC[1000]