Difference between revisions of "Part:BBa K4361117"

Revision as of 09:03, 11 October 2022

pSB1C3 with GHB / GBL reporter, deletion in Blc operator

This part shows the linearized sequence of pSB1C3 with the mutated GHB / GBL reporter system Part:BBa_K4361116, containing a single deletion when compared to the original (Part:BBa_K4361115). This mutation is expected to disrupt BlcR binding to the operator, meaning the protein cannot inhibit downstream gene expression. As the remainder of the reporter system remains intact, this part may act as a negative control against Part:BBa_K4361115 in experiments where BlcR's binding properties are measured.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2029
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2029
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2029
Illegal XhoI site found at 1013
Illegal XhoI site found at 1905
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2029
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2029
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 2155

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4361117 short</partinfo>
-This part shows the linearized sequence of pSB1C3 with the mutated GHB / GBL reporter system [[Part:BBa_K4361116]], containing a single deletion when compared to the original ([[Part:BBa_K4361115]]). This mutation is expected to disrupt BlcR binding to the operator, meaning the protein cannot inhibit downstream gene expression. As the remainder of the reporter system remains intact, this part, when inserted in plasmid pSB1C3 (see [[Part:BBa_K4361117]]), may act as a negative control against [[Part:BBa_K4361115]] in experiments where BlcR's binding properties are measured.
+This part shows the linearized sequence of pSB1C3 with the mutated GHB / GBL reporter system [[Part:BBa_K4361116]], containing a single deletion when compared to the original ([[Part:BBa_K4361115]]). This mutation is expected to disrupt BlcR binding to the operator, meaning the protein cannot inhibit downstream gene expression. As the remainder of the reporter system remains intact, this part may act as a negative control against [[Part:BBa_K4361115]] in experiments where BlcR's binding properties are measured.
 <!-- Add more about the biology of this part here