Difference between revisions of "Part:BBa K4361117"

 
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<partinfo>BBa_K4361117 short</partinfo>
 
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TBD
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This part shows the linearized sequence of pSB1C3 with the mutated GHB / GBL reporter system [[Part:BBa_K4361116]], containing a single deletion when compared to the original ([[Part:BBa_K4361115]]). This mutation is expected to disrupt BlcR binding to the operator, meaning the protein cannot inhibit downstream gene expression. As the remainder of the reporter system remains intact, this part, when inserted in plasmid pSB1C3 (see [[Part:BBa_K4361117]]), may act as a negative control against [[Part:BBa_K4361115]] in experiments where BlcR's binding properties are measured.
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K4361117 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4361117 SequenceAndFeatures</partinfo>
  

Revision as of 09:03, 11 October 2022


pSB1C3 with GHB / GBL reporter, deletion in Blc operator

This part shows the linearized sequence of pSB1C3 with the mutated GHB / GBL reporter system Part:BBa_K4361116, containing a single deletion when compared to the original (Part:BBa_K4361115). This mutation is expected to disrupt BlcR binding to the operator, meaning the protein cannot inhibit downstream gene expression. As the remainder of the reporter system remains intact, this part, when inserted in plasmid pSB1C3 (see Part:BBa_K4361117), may act as a negative control against Part:BBa_K4361115 in experiments where BlcR's binding properties are measured.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2029
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2029
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2029
    Illegal XhoI site found at 1013
    Illegal XhoI site found at 1905
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2029
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2029
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2155