Difference between revisions of "Part:BBa K4361116"

Revision as of 08:56, 11 October 2022

GHB / GBL biosensor reporter, deletion in Blc operator

This part shows the GHB / GBL reporter system designed by the Bielefeld-CeBiTec iGEM 2015 team (Part:BBa_K1758376) after site-directed mutagenesis with primers Part:BBa_K4361112 and Part:BBa_K4361113, resulting in a deletion in the Blc operator sequence. This mutation is expected to disrupt BlcR binding to the operator, meaning the protein cannot inhibit downstream gene expression. As the remainder of the reporter system remains intact, this part, when inserted in plasmid pSB1C3 (see Part:BBa_K4361117), may act as a negative control against Part:BBa_K4361115 in experiments where BlcR's binding properties are measured.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 121

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 <partinfo>BBa_K4361116 short</partinfo>
-This part shows the GHB / GBL reporter system designed by the Bielefeld-CeBiTec iGEM 2015 team ([[Part:BBa_K1758376]]) after site-directed mutagenesis with primers [[Part:BBa_K4361112]] and [[Part:BBa_K4361113]], resulting in a mutation in the Blc operator sequence. This mutation is expected to disrupt BlcR binding to the operator, meaning the protein cannot inhibit downstream gene expression. As the remainder of the reporter system remains intact, this part, when inserted in plasmid pSB1C3 (see [[Part:BBa_K4361117]]), may act as a negative control against [[Part:BBa_K4361115]] in experiments where BlcR's binding properties are measured.
+This part shows the GHB / GBL reporter system designed by the Bielefeld-CeBiTec iGEM 2015 team ([[Part:BBa_K1758376]]) after site-directed mutagenesis with primers [[Part:BBa_K4361112]] and [[Part:BBa_K4361113]], resulting in a deletion in the Blc operator sequence. This mutation is expected to disrupt BlcR binding to the operator, meaning the protein cannot inhibit downstream gene expression. As the remainder of the reporter system remains intact, this part, when inserted in plasmid pSB1C3 (see [[Part:BBa_K4361117]]), may act as a negative control against [[Part:BBa_K4361115]] in experiments where BlcR's binding properties are measured.
 <!-- Add more about the biology of this part here