Difference between revisions of "Part:BBa K4361105"

 
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<partinfo>BBa_K4361105 short</partinfo>
 
<partinfo>BBa_K4361105 short</partinfo>
  
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TBD
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Linearized sequence of the pET-11a expression plasmid. This part does not represent the full sequence of pET-11a, but rather the plasmid after digestion with BamHI as to allow for the insertion of a new DNA sequence at the cleavage site (see [[Part:BBa_K4361106]]). The T7 promoter on the plasmid allows for the transcription of the insert when the plasmid is added to a PURE reaction.
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K4361105 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4361105 SequenceAndFeatures</partinfo>
  

Revision as of 08:26, 11 October 2022


pET-11a

Linearized sequence of the pET-11a expression plasmid. This part does not represent the full sequence of pET-11a, but rather the plasmid after digestion with BamHI as to allow for the insertion of a new DNA sequence at the cleavage site (see Part:BBa_K4361106). The T7 promoter on the plasmid allows for the transcription of the insert when the plasmid is added to a PURE reaction.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 316
    Illegal XbaI site found at 5594
    Illegal PstI site found at 1068
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 316
    Illegal NheI site found at 5639
    Illegal PstI site found at 1068
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 316
    Illegal BglII site found at 5528
    Illegal BamHI site found at 5672
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 316
    Illegal XbaI site found at 5594
    Illegal PstI site found at 1068
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 316
    Illegal XbaI site found at 5594
    Illegal PstI site found at 1068
    Illegal NgoMIV site found at 3394
    Illegal NgoMIV site found at 3748
    Illegal NgoMIV site found at 3908
    Illegal NgoMIV site found at 5496
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1242
    Illegal SapI.rc site found at 2324