Difference between revisions of "Part:BBa K4365021:Design"

(→‎Source)
(→‎Design Notes)
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
yeast
+
...
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Latest revision as of 08:25, 11 October 2022


SP-SUMO-turboRFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2168
    Illegal BamHI site found at 1007
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
    Illegal NgoMIV site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

...

Source

Construct was ordered from IDT as gBlock, cloned into the p426 plasmid and fused to the N-terminus of the turboRFP fluorescent protein.

References