Difference between revisions of "Part:BBa K4365021"
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* terminator tCYC1 [https://parts.igem.org/Part:BBa_K849009 K849009]
 
* terminator tCYC1 [https://parts.igem.org/Part:BBa_K849009 K849009]
  
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[[BBa K4365021 schematic.png|500px|center|thumb|Figure 1: illustration of SP-SUMO system for secretion and scar-less protein production in yeast. The SP-SUMO is attached to the cargo protein turboRFP as this was the construct we tested during our experiments. Created with Biorender.com.]]
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[[File:BBa K4365021 schematic.png|700px|center|thumb|Figure 1: illustration of SP-SUMO system for secretion and scar-less protein production in yeast. The SP-SUMO is attached to the cargo protein turboRFP as this was the construct we tested during our experiments. Created with Biorender.com.]]
  
 
<br><span class='h3bb'>Sequence and Features</span>
 
<br><span class='h3bb'>Sequence and Features</span>

Revision as of 08:24, 11 October 2022


SP-SUMO-turboRFP

SP-SUMO-tRFP is a reporter device which could be used to test protein secretion in yeast. Moreover, if tRFP is exchanged for the protein of interest, after protein purification with His-tag you can scarlessly remove the tag from the protein with a SUMO protease. This part is optimized for expression in Saccharomyces cerevisiae.

SP-SUMO-tRFP consists of:


Figure 1: illustration of SP-SUMO system for secretion and scar-less protein production in yeast. The SP-SUMO is attached to the cargo protein turboRFP as this was the construct we tested during our experiments. Created with Biorender.com.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2168
    Illegal BamHI site found at 1007
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
    Illegal NgoMIV site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology