Difference between revisions of "Part:BBa K4164014"
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<partinfo>BBa_K4164014 short</partinfo>
 
<partinfo>BBa_K4164014 short</partinfo>
  
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In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we also tried T7<em>lac</em> promoter from pET-29a+(Novagen).
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In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we also tried T7<em>lac</em> promoter from pET-29a+(Novagen).
  
 
The composite part can be directly imported into plasmid and express FXR-ddRFPA1 induced with IPTG.
 
The composite part can be directly imported into plasmid and express FXR-ddRFPA1 induced with IPTG.

Revision as of 08:23, 11 October 2022


Inductive expression of FXR-ddRFPA1

In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we also tried T7lac promoter from pET-29a+(Novagen).

The composite part can be directly imported into plasmid and express FXR-ddRFPA1 induced with IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1558
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1147