Difference between revisions of "Part:BBa K4147012"

Revision as of 08:20, 11 October 2022

Up part of cassette for siRNA production of RXLR gene for P. capsici

Upper part of the cassette for siRNA expression. This part is part of the construct expressing the siRNA flanking the mRNA of the RXLR gene of P. capsici. It is cmpossed of a reverse T7 terminator with a RBS (BBa_K4147015) and a T7 promotor and RBS (BBa_K525998).

Usage and Biology

To produce the double-stranded siRNAs, an expression system has been designed and must be assembled. The assembly method is based on the one shown by the iGEM Ecuador 2021 team.

The figure below shows the assembly strategy that is planned to be used.

Assembly strategy for siRNA production

Sequence and Features