Difference between revisions of "Part:BBa K4365021"
Svetlana ia (Talk | contribs) |
Svetlana ia (Talk | contribs) |
||
| Line 7: | Line 7: | ||
SP-SUMO-tRFP consists of: | SP-SUMO-tRFP consists of: | ||
* FIG1 promoter [https://parts.igem.org/Part:BBa_K4365000 K4365000] | * FIG1 promoter [https://parts.igem.org/Part:BBa_K4365000 K4365000] | ||
| − | * signal peptide (alpha mating | + | * signal peptide (alpha mating factor signal peptide) [https://parts.igem.org/Part:BBa_K4365019 K4365019] |
* glycine linker [https://parts.igem.org/Part:BBa_K4365005 K4365005] | * glycine linker [https://parts.igem.org/Part:BBa_K4365005 K4365005] | ||
* 6x His-Tag [https://parts.igem.org/Part:BBa_K3033006 K3033006] | * 6x His-Tag [https://parts.igem.org/Part:BBa_K3033006 K3033006] | ||
| Line 16: | Line 16: | ||
<br><span class='h3bb'>Sequence and Features</span> | <br><span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4365021 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4365021 SequenceAndFeatures</partinfo> | ||
| + | <br> | ||
__TOC__ | __TOC__ | ||
Revision as of 08:17, 11 October 2022
SP-SUMO-turboRFP
SP-SUMO-tRFP is a reporter device which could be used to test protein secretion in yeast. Moreover if tRFP is exchanged for the protein of interest after protein purification with His-tag you scarlessly remove the tag from the protein with a SUMO protease. This part is optimized for expression in Saccharomyces cerevisiae.
SP-SUMO-tRFP consists of:
- FIG1 promoter K4365000
- signal peptide (alpha mating factor signal peptide) K4365019
- glycine linker K4365005
- 6x His-Tag K3033006
- SUMO K4365004
- codon optimized turboRFP K4365020
- terminator tCYC1 K849009
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1001
Illegal PstI site found at 1036
Illegal PstI site found at 1335 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1001
Illegal PstI site found at 1036
Illegal PstI site found at 1335 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2168
Illegal BamHI site found at 1007 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1001
Illegal PstI site found at 1036
Illegal PstI site found at 1335 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1001
Illegal PstI site found at 1036
Illegal PstI site found at 1335
Illegal NgoMIV site found at 172 - 1000COMPATIBLE WITH RFC[1000]