Difference between revisions of "Part:BBa K4139000:Design"

Revision as of 08:16, 11 October 2022

mcyH ABC Transporter for Microcystin Production

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 744
Illegal AgeI site found at 68
Illegal AgeI site found at 1132
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Codon-optimized for use in E.coli, BioBrick assembly standard

Source

Microcystis Aeruginosa mcyH gene

References

Pearson LA, Hisbergues M, Börner T, Dittmann E, Neilan BA. Inactivation of an ABC transporter gene, mcyH, results in loss of microcystin production in the cyanobacterium Microcystis aeruginosa PCC 7806. Appl Environ Microbiol. 2004 Nov;70(11):6370-8. doi: 10.1128/AEM.70.11.6370-6378.2004. PMID: 15528494; PMCID: PMC525210.

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	===References===		===References===
		+	Pearson LA, Hisbergues M, Börner T, Dittmann E, Neilan BA. Inactivation of an ABC transporter gene, mcyH, results in loss of microcystin production in the cyanobacterium Microcystis aeruginosa PCC 7806. Appl Environ Microbiol. 2004 Nov;70(11):6370-8. doi: 10.1128/AEM.70.11.6370-6378.2004. PMID: 15528494; PMCID: PMC525210.