Difference between revisions of "Part:BBa K4165019"
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A flexible 8 aa Glycine-Serine linker
 
A flexible 8 aa Glycine-Serine linker
  
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===Usage and Biology===
 
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A flexible linker with G and S aa residues with variable lengths. According to our project, 3 flexible linker required one for our clamps for binding of Tau or amyloid beta with our binding peptides . Second one for flexible linking between clamps and HTRA1 binding peptide . last one for binding our HTRA1 binding peptide with PDZ domain of our protease HTRA1. our linker with length [8 aa GGSGGGGG ] was calculated by our team for testing how it would affect interaction of our mentioned parts with each other using Pymol Software.
 
  
 
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===<span class='h3bb'>Sequence and Features</span>===
 
===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K4165019 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165019 SequenceAndFeatures</partinfo>
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===Dry Lab Characterization===
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In our project, we generated a library for GS flexible linkers to be tested on different parts of our system; each linker was modeled in the fusion protein, and we filtered through them until we found the suitable length to use in linking our PROTAC, clamps, and switches together.
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Revision as of 07:58, 11 October 2022


GGSGGGGG Linker

A flexible 8 aa Glycine-Serine linker


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Dry Lab Characterization

In our project, we generated a library for GS flexible linkers to be tested on different parts of our system; each linker was modeled in the fusion protein, and we filtered through them until we found the suitable length to use in linking our PROTAC, clamps, and switches together.