Difference between revisions of "Part:BBa K4164996:Design"
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
This sequence is composed of BBa_J04450, BBa_K3139011 and BBa_K4164007.  
+
This sequence is composed of [https://parts.igem.org/Part:BBa_J04450 BBa_J04450], [https://parts.igem.org/Part:BBa_K3139011 BBa_K3139011] and [https://parts.igem.org/Part:BBa_K4164007 BBa_K416007].
  
 
===References===
 
===References===

Latest revision as of 07:57, 11 October 2022


mRFP-TEVsite-Ssr


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The stop codon of the BBa_J04450 was deleted and TEV protease cutting site and Ssr tag were added to the C-terminus of mRFP by homologous recombination.


Source

This sequence is composed of BBa_J04450, BBa_K3139011 and BBa_K416007.

References