Difference between revisions of "Part:BBa K4164997:Design"
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
This sequence is composed of [https://parts.igem.org/Part:BBa_K4164003 BBa_K416003] and [https://parts.igem.org/Part:BBa_K4164005 BBa_K416005].
+
This sequence is composed of [https://parts.igem.org/Part:BBa_K4164003 BBa_K416003]and [https://parts.igem.org/Part:BBa_K4164005 BBa_K416005].
  
  
 
===References===
 
===References===

Latest revision as of 07:50, 11 October 2022


RXR-3*GS Linker-ddRFPB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1867
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 357
    Illegal AgeI site found at 1384
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 739


Design Notes

The stop codon of the RXR was deleted and a flexible linker (3*GGGGS) was added between RXR and ddRFPB1.


Source

This sequence is composed of BBa_K416003and BBa_K416005.


References