Difference between revisions of "Part:BBa K4164997:Design"

Line 13: Line 13:
 
===Source===
 
===Source===
  
This sequence is composed of [https://parts.igem.org/Part:BBa_K4164003
+
This sequence is composed of [https://parts.igem.org/Part:BBa_K4164013 BBa_K528004]
BBa_K4164003] and [https://parts.igem.org/Part:BBa_K4164005
+
BBa_K4164005]
+
 
+
  
 +
https://parts.igem.org/wiki/index.php?title=Part:BBa_K528004
 +
https://parts.igem.org/Part:BBa_K4164013
 
===References===
 
===References===

Revision as of 07:46, 11 October 2022


RXR-3*GS Linker-ddRFPB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1867
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 357
    Illegal AgeI site found at 1384
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 739


Design Notes

The stop codon of the RXR was deleted and a flexible linker (3*GGGGS) was added between RXR and ddRFPB1.


Source

This sequence is composed of BBa_K528004

https://parts.igem.org/wiki/index.php?title=Part:BBa_K528004 https://parts.igem.org/Part:BBa_K4164013

References