Difference between revisions of "Part:BBa K4271004"
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The pNP Mut 1-1 is a gene that encodes the activation protein for downstream GFP expressions. After adding pNP to the sensor plasmid BL21, it binds to the pNP Mut 1-1 protein and acts as a transcriptional factor for GFP. In our experiment, we measure the level of GFP fluorescence to determine the success of paraoxon degradation. After the pNP Mut 1-1 protein is used, the pNP Mut 1-1 gene is expressed again, continuing the cycle.   
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The pNP Mut 1-1 is a gene that encodes the activation protein for downstream GFP expressions. As the pNP (p-nitrophenol) in the environment increases, pNP molecule binds to the pNP Mut 1-1 protein and acts as a transcriptional factor for GFP. In our experiment, we measure the level of GFP fluorescence to determine the success of paraoxon degradation. After the pNP Mut 1-1 protein is used, the pNP Mut 1-1 gene is expressed again, continuing the cycle.   
  
 
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Revision as of 07:23, 11 October 2022


pNP Mut 1-1

The pNP Mut 1-1 is a gene that encodes the activation protein for downstream GFP expressions. As the pNP (p-nitrophenol) in the environment increases, pNP molecule binds to the pNP Mut 1-1 protein and acts as a transcriptional factor for GFP. In our experiment, we measure the level of GFP fluorescence to determine the success of paraoxon degradation. After the pNP Mut 1-1 protein is used, the pNP Mut 1-1 gene is expressed again, continuing the cycle.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 672
  • 1000
    COMPATIBLE WITH RFC[1000]