Difference between revisions of "Part:BBa K4275000"
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<partinfo>BBa_K4275000 short</partinfo> | <partinfo>BBa_K4275000 short</partinfo> | ||
| − | + | K.lactis mα is a α-mating secretion signal in Kluyveromyces lactis that encodes for a α-mating factor (αMF) domain fusing with desired protein. The signal peptide in αMF domain expressed direct the fusion protein into endoplasmic reticulum (ER), Golgi body and thus secrete in vitro.K.lactis αMF is integrated upstream of gene of interest (e.g FAST-PETase) to express a αMF domain fused with desired protein (enzymes e.g PETase), which direct the enzyme to secrete from the host cell. The design eliminates process of lysing host cell and purifying desired proteins therefore reduce the cost of the whole textile degradation process. | |
| + | The integrated part αMF also provides a inspiration of extracellular protein secretion for future iGEM teams, paving the way of improving the efficiency of their protein secretion system. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4275000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4275000 SequenceAndFeatures</partinfo> | ||
Revision as of 07:02, 11 October 2022
Kluyveromyces marxianus alpha mating-factor secretion signal
K.lactis mα is a α-mating secretion signal in Kluyveromyces lactis that encodes for a α-mating factor (αMF) domain fusing with desired protein. The signal peptide in αMF domain expressed direct the fusion protein into endoplasmic reticulum (ER), Golgi body and thus secrete in vitro.K.lactis αMF is integrated upstream of gene of interest (e.g FAST-PETase) to express a αMF domain fused with desired protein (enzymes e.g PETase), which direct the enzyme to secrete from the host cell. The design eliminates process of lysing host cell and purifying desired proteins therefore reduce the cost of the whole textile degradation process. The integrated part αMF also provides a inspiration of extracellular protein secretion for future iGEM teams, paving the way of improving the efficiency of their protein secretion system.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]