Difference between revisions of "Part:BBa K4221007"

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===Design Consideration===
 
===Design Consideration===
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The construct was cloned into a PET28a plasmid and transformed into RGD-PET28a [3]
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The construct was cloned into a PET28a plasmid and transformed into BL21 (DE3) E. coli.
  
 
The construction includes:
 
The construction includes:

Latest revision as of 06:35, 11 October 2022


RGD

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage

Near-infrared (NIR) fluorescent probes have become powerful tools for non-invasive monitoring of various biologically important processes in vivo. To meet the requirement of improving the function of NIR, one has to find a material that can render NIR fluorescent probes soluble and selective simultaneously. The hybrid hydrophobin-RGD fusion protein preserving dual abilities can render NIR fluorescent probes soluble and selective simultaneously.

Our team is trying to improve self-assembly and targeting functions from HFBI and RGD peptide and replacing HFBI with BslA.[2]

Biology

RGD is a tripeptide sequence containing arginine-glycine-aspartate, which is a recognition site for the interaction between integrins and their ligands. It mediates the adhesion between cells and extracellular matrix and between cells, and has signal transduction function, thereby mediating many important life activities.[1]

Design Consideration

The construct was cloned into a PET28a plasmid and transformed into BL21 (DE3) E. coli.

The construction includes:

RGD is fused with BslA with a GS linker（GGTGGTGGCGGCAGCGGCGGAGGCGGTAGT）

Reference

[1]Qu Hong, WangXiangcheng, Wang Cheng, etal. Research progress and clinical application of RGD peptide[J]. Journal Of Inner Mongolia Medical University, 2019, 41(6):660-663.

[2]Xiao Y, Zhang Q, Wang Y. Dual-functional protein for one-step production of a soluble and targeted fluorescent dye[J]. Theranostics, 2018, 8(11):3111-3125.

[3] Aijia J, Xibin N. Construction and Expression of Prokaryotic Expression Vector pET28a-EGFP[J]. JOURNAL OF MICROBIOLOGY, 2011, 31(4):69-73.