Difference between revisions of "Part:BBa K4324300"

 
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<partinfo>BBa_K4324300 short</partinfo>
 
<partinfo>BBa_K4324300 short</partinfo>
  
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Xylose Reductase (XR)
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This part is a collection of all composite parts for xylose reductase ([https://parts.igem.org/Part:BBa_K4324000 BBa_K4324000]), xylitol dehydrogenase ([https://parts.igem.org/Part:BBa_K4324001 BBa_K4324001]), xylulose kinase ([https://parts.igem.org/Part:BBa_K4324002 BBa_K4324002]), and phosphoketolase ([https://parts.igem.org/Part:BBa_K4324003 BBa_K4324003]). This part is a collection of all composite parts within '''TheKingsSchool_AU_HS''''s project.
  
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Xylose Dehydrogenase (XDH)
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This part enables E. coli to express the XR-XDH pathway, further express XK, and express phosphoketolase (links xylulose-5-phosphate to glycolysis).
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Xylose Kinase (XK)
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Phosphoketolase (PK)
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4324300 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4324300 SequenceAndFeatures</partinfo>
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==Xylose Reductase (XYL1)==
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==Xylitol Dehydrogenase (XYL2)==
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==Xylulose Kinase (xylB) ==
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==Phosphoketolase (XFP)==
  
  

Revision as of 06:32, 11 October 2022


XR-XDH-XK-PK for E. coli

This part is a collection of all composite parts for xylose reductase (BBa_K4324000), xylitol dehydrogenase (BBa_K4324001), xylulose kinase (BBa_K4324002), and phosphoketolase (BBa_K4324003). This part is a collection of all composite parts within TheKingsSchool_AU_HS's project.

This part enables E. coli to express the XR-XDH pathway, further express XK, and express phosphoketolase (links xylulose-5-phosphate to glycolysis).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1649
    Illegal BglII site found at 4065
    Illegal BamHI site found at 4362
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5767
    Illegal AgeI site found at 2929
    Illegal AgeI site found at 3217
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1306
    Illegal SapI.rc site found at 747

Xylose Reductase (XYL1)

Xylitol Dehydrogenase (XYL2)

Xylulose Kinase (xylB)

Phosphoketolase (XFP)