Difference between revisions of "Part:BBa K176098:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K176098 short</partinfo> | <partinfo>BBa_K176098 short</partinfo> | ||
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pCon 0.70(J23101) generates LuxR. | pCon 0.70(J23101) generates LuxR. | ||
− | pLux/Tet(K176000) generates ccdB to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc. | + | pLux/Tet(K176000) generates lacZalpha-ccdB to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc. |
Latest revision as of 05:07, 8 October 2009
Cell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 856
Illegal NheI site found at 879 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 698
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2323
Illegal BsaI.rc site found at 1834
Design Notes
Input: AHL
Output1: AHL
Output2: Cell Density
Design:
pCon 0.01(J23103) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.
pCon 0.70(J23101) generates LuxR.
pLux/Tet(K176000) generates lacZalpha-ccdB to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc.
Source
K176087 K176063