Difference between revisions of "Part:BBa K4317001"

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Microorganisms 2020, 8, 1030; doi:10.3390/microorganisms8071030
 
Microorganisms 2020, 8, 1030; doi:10.3390/microorganisms8071030
  
 
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https://static.igem.org/mediawiki/parts/thumb/2/24/P2069M.png/800px-P2069M.png
 
<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
 
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https://static.igem.org/mediawiki/parts/thumb/5/51/Promoter_strength.png/800px-Promoter_strength.png
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 05:30, 11 October 2022


P2069M

P2069 originated from B. pumilus BA06. P2069M, engineered by Cui-Cui Miao and colleagues, has strong promoter activity in Bacillus subtilis.

P2069M_ref.png Microorganisms 2020, 8, 1030; doi:10.3390/microorganisms8071030

800px-P2069M.png Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal BglII site found at 13
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]