Difference between revisions of "Part:BBa K238015:Design"

 
(→‎Design Notes)
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K238015 short</partinfo>
 
<partinfo>BBa_K238015 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
none
+
This part was actually put in a pBR322 vector.  This is a vector which has a low copy number (around 15-20). This is needed to make the promoter work properly in E.Coli. The low copy number ensures a good ratio between the promoter and its repressor, which is naturally present in E.Coli. When a high copy number is used, the promoter is abundant which results in a constituitivly active promoter.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Latest revision as of 15:55, 7 October 2009

blue light promoter and GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 757


Design Notes

This part was actually put in a pBR322 vector. This is a vector which has a low copy number (around 15-20). This is needed to make the promoter work properly in E.Coli. The low copy number ensures a good ratio between the promoter and its repressor, which is naturally present in E.Coli. When a high copy number is used, the promoter is abundant which results in a constituitivly active promoter.

Source

the promotor comes from the genome of E.coli MC4100

References