Difference between revisions of "Part:BBa K4169015"
m |
m |
||
Line 12: | Line 12: | ||
<html> | <html> | ||
− | <div class = "center"><center><img src = "https://static.igem.wiki/teams/4169/wiki/backword/dma/tmd-dmd-structure/tmadh1-1.png" style = "width: | + | <div class = "center"><center><img src = "https://static.igem.wiki/teams/4169/wiki/backword/dma/tmd-dmd-structure/tmadh1-1.png" style = "width:75%"></center><br></div> |
</html> | </html> | ||
Revision as of 03:18, 11 October 2022
produce TMADH
After expressing, it'll produce trimethylamine dehydrogenase (TMADH (EC 1.5.99.7)). The enzyme TMADH is an iron–sulfur flavoprotein which catalyses the oxidative demethylation of trimethylamine (TMA) to dimethylamine and formaldehyde:
(CH3)3N + H2O → (CH3)2NH + CH2O +2H+ + 2e-.
Metabolic Pathway
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 388
Illegal PstI site found at 183
Illegal PstI site found at 1782 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 388
Illegal PstI site found at 183
Illegal PstI site found at 1782 - 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 388
Illegal PstI site found at 183
Illegal PstI site found at 1782 - 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]